Nuclease-free water means water free from both RNase and DNase enzymes. The presence of these enzymes can degrade the molecular samples. Methods of removing nucleases include treating with DEPC (diethylpyrocarbonate), filtration, and autoclaving. Nuclease-free water is used in applications such as PCR, sequencing, cloning, cDNA synthesis and nucleic acid purification.
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We have already studied the definitions of chromosome, genome, and DNA in the previous blogs. Now, let us have a look on what is there insid...
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